Adição de extrato de açaí em diferentes proporções no meio diluente para a criopreservação do sêmen ovino

Abstract

Semen cryopreservation for the application of assisted reproduction biotechnics has been very important for genetic use. However, this process undergoes several influences, including the diluter composition and the excessive production of reactive oxygen species, generating oxidative stress in the cell. Açaí, in turn, has antioxidant capacity attributed to its polyphenolic fraction, rich in anthocyanins, which act on the diphenylpicrylhydrazyl (DPPH) radical, as well as on the superoxide and hydroxyl radicals. Thus, the objective was to evaluate the effect of adding açaí extract to sheep semen freezing extender on the kinetics, morphology and integrity of the sperm plasma membrane. Semen samples were collected from five Dorper sheep, divided into 0% control treatments an inclusion of 5 and 10% of açaí extract. Thus, sperm kinetics did not show significant differences, as well as plasma membrane integrity. Therefore, the addition of açaí extract to the extender does not affect the quality of the cryopreserved semen.